David Sinclair Supplements 2025: Full Stack Overview

If you’ve ever wondered, “What supplements does David Sinclair actually take, and why does he choose that specific dose?”, this guide breaks down the core ingredients and the science behind them. More importantly, it provides the parameters you need to select the right supplements and formulation for your own evidence-based longevity stack.

In short: if you choose one supplement, focus on an NAD+ booster like NMN. However, a true strategy goes beyond a basic boost: it requires maintaining those elevated levels and simultaneously improving the crucial cellular repair processes that use NAD+.

Dr. David A. Sinclair, a Harvard professor of genetics and author of the bestselling book Lifespan, is one of the most influential voices in ageing and longevity science.

His pioneering research in epigenetics and cellular repair has reframed ageing as a biological process that can potentially be slowed, halted, or even reversed, rather than an inevitable decline.

Sinclair’s personal supplement routine has since become a model for biohackers, longevity enthusiasts, and researchers alike. His approach targets the hallmarks of ageing—from mitochondrial decline to genomic instability—using nutrients that support cellular energy, repair, and resilience.

According to Dr. Sinclair, ageing is not a mystery, it’s a treatable biological condition caused by damage to cellular systems over time.

His research identifies key molecules, such as NMN (nicotinamide mononucleotide) and resveratrol, as potential tools to restore youthful cellular function.

These two compounds form the core of his supplement stack, working synergistically to:

• Boost NAD+ (cellular energy coenzyme)

• Activate sirtuins (longevity-regulating proteins)

• Support DNA repair and stress resistance

NMN is a direct precursor to NAD+, a molecule essential for energy metabolism, DNA repair, and mitochondrial function.

As we age, NAD+ levels decline—contributing to fatigue, inflammation, and metabolic slowdown. Sinclair’s research suggests that restoring NAD+ with NMN can help counteract these effects.

Studies in older mice have shown that NMN supplementation:

• Improves mitochondrial function

• Enhances physical endurance

• Restores youthful gene expression patterns

Sinclair has publicly said he takes NMN every morning. His widely discussed personal dosage is approximately 1 gram per day, taken with water.

While Dr. Sinclair's personal NMN dose is 1 gram (1000 mg), this is notably higher than the doses used in most published human clinical trials, which often show significant biological benefits at 250 mg to 500 mg daily. 

The rationale for the higher 1g dose stems from historical mouse-to-human extrapolation models and a desire to ensure maximal NAD+ saturation, especially given historical challenges with NMN's bioavailability in standard capsules. However, newer studies suggest that due to efficiency and cost, a dose in the 400 mg – 600 mg range may be sufficient to reach near-maximal systemic NAD+ increases for many individuals.

For Youth Recommended Dosage & Formulation: While Dr. Sinclair takes higher doses, our formulation is carefully calibrated. We recommend a daily dose of 450 mg, aligning with stringent regulatory compliance guidelines in regions like Singapore (HSA). Furthermore, our premium formula, The Repair, is a comprehensive stack that includes Apigenin (a bioflavanoid known to preserve the elevated NAD+ levels) and Pterostilbene (a highly bioavailable sirtuin activator), effectively boosting the impact of the NMN by keeping cellular energy high and activating repair pathways simultaneously.

Choosing a reliable NMN source is paramount. When considering a supplement, prioritise brands that use clinically studied ingredients like Uthever® NMN and provide transparent, third-party testing for purity and consistency.

We did the hard work for you, find out which brand sells the best NMN in your country:

Best known as the “red wine molecule,” resveratrol is a polyphenol that acts as a potent activator of sirtuins (SIRT1), a family of longevity-regulating proteins.

Dr. Sinclair famously takes his resveratrol with yogurt or healthy fats to enhance absorption. (Note: His typically discussed personal dosage is approximately 1 gram per day, often taken alongside NMN.)

When combined with NMN, the two compounds create a powerful cellular repair pathway. Resveratrol acts as the  switch that turns on the sirtuin repair crew (SIRT1). However, sirtuins cannot function without sufficient  fuel, which is NAD+. By taking NMN (the NAD+ precursor) alongside Resveratrol, the body ensures the activated sirtuins have the necessary energy coenzyme to execute DNA repair, stress  defence, and metabolic  optimisation, mimicking some effects of calorie restriction.

While Resveratrol is the most studied Sirtuin-Activating Compound (STAC), its poor bioavailability (short half-life, rapid metabolism) means high doses are often required for efficacy. This is why many researchers and formulators favour Pterostilbene, a superior alternative.

Pterostilbene (included in our The Repair formula) is a highly bioavailable sirtuin activator known for its greater metabolic stability, boasting a half-life of over 100 minutes, significantly longer than Resveratrol's 14 minutes.

Due to this enhanced efficiency, just 50 mg of Pterostilbene in our formula is considered an efficacious dose equivalent to 500mg to 1,000mg of standard Resveratrol, delivering sustained biological activation of the SIRT1 pathway.

The most advanced longevity protocols go beyond NAD+ and sirtuin activation to address other critical hallmarks of ageing: senolysis (clearing old cells) and autophagy (recycling cellular components). Sinclair's extended stack includes complementary compounds for these functions:

• Quercetin (Senolysis): An important flavonoid used for senolytic activity—it helps the body identify and clear aged, damaged cells (senescent cells) that otherwise cause chronic low-grade inflammation.

• Spermidine (Autophagy): A naturally occurring polyamine that promotes autophagy (cellular renewal processes), essentially clearing out damaged or unnecessary cellular components to ensure healthy function.

• Fisetin (Senolytic/Sirtuin Support): A potent flavonoid that, like Quercetin, exhibits strong senolytic properties. Fisetin also acts as a sirtuin activator and may cross the blood-brain barrier more easily, making it a powerful multi-functional compound.

• Grape Seed Extract (Vascular Support): These compounds (Proanthocyanidins), related to Resveratrol, are potent polyphenols that primarily support cardiovascular health and cellular signalling pathways, offering broad antioxidant  defence.

• N,N-dimethylbiguanide: (Under medical supervision) – A prescription drug used to support metabolic flexibility, though studies on its longevity benefits in humans are ongoing.

These compounds work to support the body’s innate ability to repair itself and actively delay biological ageing through multiple pathways.

David Sinclair’s approach isn’t about chasing trends—it’s about understanding the mechanisms of ageing and supporting the body’s natural ability to repair and rejuvenate.

To create a Sinclair-inspired stack focused on cellular repair:

1. Start with an NAD+ booster: Select a high-quality NMN supplement to restore cellular fuel.

2. Add a Sirtuin Activator: Combine NMN with Resveratrol or a highly bioavailable alternative like Pterostilbene for synergistic sirtuin activation.

3. Support Autophagy and Senolysis: Introduce compounds like spermidine and quercetin for enhanced cellular housekeeping.

4. Prioritise Lifestyle: Supplements are adjunctive. Focus first on lifestyle cornerstones: exercise, intermittent fasting, and a low-sugar diet—all of which Sinclair practices.

Dr. David Sinclair’s supplement regimen is not a “quick fix.” It’s a science-based framework built around repairing, protecting, and  optimising the body at the cellular level. By focusing on NAD+ restoration, sirtuin activation, and cellular renewal, his stack represents the future of proactive, data-driven health.